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cDNA library: NICHD_XGC_Tad1

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Information for 1 library

whole body

   1. NICHD_XGC_Tad1

NICHD_XGC_Tad1

Name: NICHD_XGC_Tad1
Library ID: 2000
Organism: Xenopus laevis
Age: 0
Organ: whole body
Tissue: developing tadpole
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.

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