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cDNA library: NIH_MGC_136

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Information for 1 library


   1. NIH_MGC_136


Name: NIH_MGC_136
Library ID: 2003
Organism: Mus musculus
Strain: CD-1
Age: 0
Stage: embryo
Organ: embryo
Tissue: limb, maxilla and mandible: 5, 4 and 1 limb and jaw equivalents from 17.5, 18.5 and newborn days respectively
Host: DH10B TonA
Vector: pCMV-SPORT6.1
Vector type: phagemid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: Normalized, full-length enriched library from pool of mouse embronic limb, maxilla and mandible, embryonic day 17.5, 18.5 and newborn (mandible (5, 4 and 1 limb and jaw equivalents from respective days). Cloned directionally, oligo-dT primed (5''-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3''. Size selected for the 1kb fragments, average insert size 1.2 kb. Normalization to Cot 7.5 . Tissue contributed by David Rowe; library constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library.

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