Name: NIH_MGC_136 Library ID: 2003 Organism: Mus musculus Strain: CD-1 Age: 0 Stage: embryo Organ: embryo Tissue: limb, maxilla and mandible: 5, 4 and 1 limb and jaw equivalents from 17.5, 18.5 and newborn days respectively Host: DH10B TonA Vector: pCMV-SPORT6.1 Vector type: phagemid Insert digest: 5' EcoRV/NotI 3' Stop Codon Status: without Description: Normalized, full-length enriched library from pool of mouse embronic limb, maxilla and mandible, embryonic day 17.5, 18.5 and newborn (mandible (5, 4 and 1 limb and jaw equivalents from respective days). Cloned directionally, oligo-dT primed (5''-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3''. Size selected for the 1kb fragments, average insert size 1.2 kb. Normalization to Cot 7.5 . Tissue contributed by David Rowe; library constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library.
