Name: NIH_MGC_165 Library ID: 2005 Organism: Mus musculus Strain: CD-1 Age: 0 Stage: juvenile Organ: testis Tissue: primary cultures of Sertoli cells isolated from testis Host: DH10B TonA Vector: pDNR-LIB Vector type: plasmid Insert digest: SfiI (directional) Stop Codon Status: without Description: 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C or G and N = A, C, G or T). Average insert size 1.4 kb (range 0.6-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.