Name: NIH_MGC_172 Library ID: 2032 Organism: Homo sapiens Age: 0 Stage: embryo Organ: embryonic stem cell Tissue: Embryonic Stem Cells H1 Host: DH10B TonA Vector: pDONR201 Vector type: plasmid Insert digest: 5' attP2/attP1 Stop Codon Status: without Description: cDNA made by oligo-dT with attB2 site and directionally cloned. Priming sequence: 5'-TTTCCTGCAGGCCGGCCACCACTTTGTACAAGAAAGCTGGGTTTTTTTTTTTTTTTTTTT-3'. Full-length enriched library was constructed using the GeneRacer kit by Invitrogen, library amplification 16 cycles. Library constucted by Mark Bittinger in the Bradfield laboratory (McArdle Laboratory for Cancer Research, University of Wisconsin). Note: this is a NIH_MGC Library.