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cDNA library: NICHD_MM_Hyp1

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Information for 1 library

brain/CNS

   1. NICHD_MM_Hyp1

NICHD_MM_Hyp1

Name: NICHD_MM_Hyp1
Library ID: 2034
Organism: Mus musculus
Strain: C57BL
Gender: both
Age: 0
Stage: mixed
Organ: brain/CNS
Tissue: normal mediobasal and hypothalamus
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5' -ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.08 kb (range 0.73-1.37 kb). 13/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

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