Name: NIH_BMAP_GH0 Library ID: 2036 Organism: Mus musculus Strain: C57BL/6 Age: 0 Stage: adult Organ: brain/CNS Tissue: whole brain, pooled Host: DH10B TonA Vector: pYX-Asc Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: Non-normalized full-length enriched library from pooled mouse brain tissue, adult days 1, 5 and 15 (size selected). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCGAACTGAATTTTTTTTTTTTTTTTTTT-3')contains the sequence tag CGAACTGAAT between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Size-selected for the 4-5 kb fraction. Library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy Project (BMAP).