Name: NIH_MGC_191 Library ID: 2039 Organism: Homo sapiens Age: 0 Stage: adult Organ: T-cell Tissue: Peripheral Blood Mononuclear Cells (PBMC), pooled Host: DH10B TonA Vector: pDNR-LIB Vector type: plasmid Insert digest: SfiI (directional) Stop Codon Status: without Description: Library is oligo-dT primed and directionally cloned. PBMC - Peripheral Blood Mononuclear Cells. RNA was pooled from 3/6 hour stimulation with PMA and Ionomycin. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.69 kb (range 0.70-5.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library.