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cDNA library: NICHD_XGC_Swb1N

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Information for 1 library

whole body

   1. NICHD_XGC_Swb1N

NICHD_XGC_Swb1N

Name: NICHD_XGC_Swb1N
Library ID: 2049
Organism: Xenopus tropicalis
Gender: male
Age: 0
Stage: adult
Organ: whole body
Tissue: Whole body
Host: DH10B TonA
Vector: pExpress-1
Vector type: plasmid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: Bulk tissue was collected from a whole 10 month old male from the F6 strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.5 kb fragments for an average insert size of 1.92 kb. Library was normalized to Cot5 with a 180-fold reduction of actin. A non-normalized version of this library is also available (NICHD_XGC_Swb1). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Xenopus Gene Collection library.

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