Name: NIH_ZGC_10 Library ID: 2052 Organism: Danio rerio Strain: Tubergin Age: 0 Stage: adult Organ: whole body Host: DH10B TonA Vector: pExpress-1 Vector type: plasmid Insert digest: 5' EcoRV/NotI 3' Stop Codon Status: without Description: Bulk tissue was collected from a whole adult individual from the Tubergin strain. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double- stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for 1 kb fragments. A normalized version of this library is also available (NIH_ZGC_7). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Zebrafish Gene Collection library.
