Name: Organ of Corti Library ID: 2056 Organism: Mus musculus Strain: BALB/c Gender: both Age: 0 Stage: juvenile Organ: inner ear Tissue: Ear - Organ of Corti (sensory hearing epithelial tissue within the inner ear). Pooled from several tissues from one or more individulas: pooled from 364 organs of Corti. Expect the following cell types to be present: inner and outer hair cells, Deiters cells, Hensen cells, inner and outer phalangeal cells. There also may be some neuronal cells, fibroblast and blood cells. Host: DH10B TonA Vector: pBluescript SK- Vector type: phagemid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: The organ of Corti (OC) was fine dissected from a total of 386 OC as follows: 102 samples from post-natal (P) day 5; 72 from P6; 60 from P7; 46 from P8; 18 from P9; 20 from P10; 14 from P12 and 24 from P13. Total RNA was extracted using the micro Fasttrack kit (catalog # K1593-02; Invitrogen, Carlsbad, CA), according to manufacturer's instructions. Reverse transcription and library construction were carried out with the Uni-Zap XR vector kit (catalog # 237211, Stratagene) and Uni-Zap XR Gigapack III Gold Cloning kit (catalog # 237612), both from Stratagene (La Jolla, CA, USA), according to manufacturer's instructions. The frequency distribution of the library is as follows: 72% of genes have 1 copy; 14.3% 2; 12% 3-10; 1.4% 11-50 and 0.1% 51-150. As to gene function, 45% of genes are present in GenBank and have know function; 23% have hits in GenBank,but do not have assigned function; 12% are uncharacterized ESTs and 20% are unidentified. Library created in the laboratory of M. Brownstein (NIMH, NIH). A complete library decription can be found at NCBI.