Name: NICHD_Hs_Ov1 Library ID: 2057 Organism: Homo sapiens Gender: female Age: 0 Stage: adult Organ: ovary Tissue: Granulosa lutein cells aspirated from preovulator follicles of normal cycling women undergoing ovulation induction of infertility due to male factor and normal doners. The cells were from follicles stimulated with Lupron, FSH and hCG. Polled tissures from Percoll purified granulosa lutein cells. Host: DH10B TonA Vector: pDNR-LIB Vector type: plasmid Insert digest: SfiI (directional) Stop Codon Status: without Description: Library is oligo-dT primed and directionally cloned. Granulosa lutein cells aspirated from preovulatory folicles of normal cycling women undergoing ovulation induction for infertility due to male factor and normal doners. The cells were from follicles stimulated with Lupron, FSH and hCG. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 2.23 kb (range 1.0-4.5 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).
