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cDNA library: NICHD_Hs_Ov1

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Information for 1 library

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   1. NICHD_Hs_Ov1

NICHD_Hs_Ov1

Name: NICHD_Hs_Ov1
Library ID: 2057
Organism: Homo sapiens
Gender: female
Age: 0
Stage: adult
Organ: ovary
Tissue: Granulosa lutein cells aspirated from preovulator follicles of normal cycling women undergoing ovulation induction of infertility due to male factor and normal doners. The cells were from follicles stimulated with Lupron, FSH and hCG. Polled tissures from Percoll purified granulosa lutein cells.
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned. Granulosa lutein cells aspirated from preovulatory folicles of normal cycling women undergoing ovulation induction for infertility due to male factor and normal doners. The cells were from follicles stimulated with Lupron, FSH and hCG. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 2.23 kb (range 1.0-4.5 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

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