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cDNA library: NIH_MGC_222

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Information for 1 library

placenta

   1. NIH_MGC_222

NIH_MGC_222

Name: NIH_MGC_222
Library ID: 2060
Organism: Mus musculus
Strain: C57BL/6
Age: 0
Stage: placental
Organ: placenta
Tissue: pool of 3 placentas from one mouse
Host: DH10B TonA
Vector: pExpress-1
Vector type: plasmid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: RNA obtained from three placentas from female C57/BL6 mouse at 16 days pregnancy. Tissues were snap-frozen and kept at -80C for two days before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5''-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1 kb resulted in an average insert size of 1.5 kb. Library is not amplified. (Normalized version of this library is NIH_MGC_203.) Library constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC Library.

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