Name: NIH_BMAP_HB0 Library ID: 2067 Organism: Mus musculus Strain: C57BL/6 Age: 0 Stage: embryo Organ: eye Tissue: pooled eye Host: DH10B TonA Vector: pYX-Asc Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: Non-normalized full-length enriched library from pooled mouse eye tissue embryonic days 12.5, 13.5 and 14.5 (size selected for 4-5 kb). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first- synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCTTATTGAAGTTTTTTTTTTTTTTTTTT-3') contains the sequence tag TTATTGAAGT between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
