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cDNA library: NCI_CGAP_EZ1

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Information for 1 library

cartilage

   1. NCI_CGAP_EZ1

NCI_CGAP_EZ1

Name: NCI_CGAP_EZ1
Library ID: 2077
Organism: Homo sapiens
Age: 0
Organ: cartilage
Tissue: chondrosarcoma, grade II
Host: DH10B TonA
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTAATATGTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (a non-normalized version of this library is also available, NCI_CGAP_EZ0). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.

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