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cDNA library: NCI_CGAP_FT1

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Information for 1 library


   1. NCI_CGAP_FT1


Library ID: 2087
Organism: Homo sapiens
Age: 0
Organ: macrophage
Tissue: alveolar macrophage, pool of 81, challenged with different treatments
Host: DH10B TonA
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGGCCATGCCGTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (a non-normalized version, NCI_CGAP_FT0, as well as a subtracted version, NCI_CGAP_FT2, of this library are also available). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.

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