I.M.A.G.E. Consortium
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cDNA library: GISZF001
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Information for 1 library
embryo
1.
GISZF001
GISZF001
Name:
GISZF001
Library ID:
2089
Organism:
Danio rerio
Strain:
wild type, Singapore strain
Age:
0
Stage:
embryo
Organ:
embryo
Tissue:
whole embryos, pooled from 7 embryonic stages
Host:
DH10B
Vector:
pDNR-LIB
Vector type:
plasmid
Insert digest:
SfiI (directional)
Stop Codon Status:
without
Description:
Priming method: Sfi-(dT)30 primed. Priming sequence: 5'- ATTCTAGAGGCCGAGGCGGCCGACATG(T)30VN-3'; directionally cloned into 5' cloning site (SfiA) using linker/adaptor sequence 5'- AAGCAGTGGTATCAACGCAGAGTGGCC-3' and 3' cloning site (SfiB) using Linker/adaptor sequence 5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30VN-3' (same as the priming sequence). Average insert size 2 kb. For PCR insert analysis use forward and reverse primers. Library amplified recombinants (inserts): 98%; library complexity:5x10E6; full-length construction method: SMART (Clontech). Library constructed by S. Mathavan, Chia-Lin Wei and Yijun Ruan (Genome Institute of Singapore).
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