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cDNA library: NIH_MGC_227

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Information for 1 library

brain/CNS

   1. NIH_MGC_227

NIH_MGC_227

Name: NIH_MGC_227
Library ID: 2090
Organism: Homo sapiens
Gender: male
Age: 47
Stage: adult
Organ: brain/CNS
Tissue: Bulk tissue from Human Spinal cord
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned. 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N-1N-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected for >0.5kb with an average insert size of 1.3kb Library created in the laboratory of Jonathan Kuo and Ted Usdin. Note: this is a NIH_MGC Library

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