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cDNA library: NIH_MGC_229

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Information for 1 library


   1. NIH_MGC_229


Name: NIH_MGC_229
Library ID: 2092
Organism: Homo sapiens
Gender: male
Age: 47
Stage: adult
Organ: brain/CNS
Tissue: Human Brain - Frontal Cortex
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned.5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)3N-1N-3. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected for >0.5kb with an average insert size of 1.2kb Library created in the laboratory of Jonathan Kuo and Ted Usdin.

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