Name: NIH_MGC_214 Library ID: 2107 Organism: Homo sapiens Age: 0 Organ: lung Tissue: pool of 3 metastases Host: DH10B TonA Vector: pYX-Asc Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: Non-normalized full-length enriched library from pooled lung metastases (cell lines) . The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCGATAAGGCCATTTTTTTTTTTTTTTTTT -3') contains the sequence tag GATAAGGCCA between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library constructed by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa). Note: this is a NIH_MGC Library.