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cDNA library: NIH_MGC_149

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Information for 1 library

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   1. NIH_MGC_149

NIH_MGC_149

Name: NIH_MGC_149
Library ID: 2110
Organism: Mus musculus
Strain: C57BL/Ka-Thy1
Age: 0
Stage: embryo
Organ: stem cell
Tissue: early hematopoietic precursor cells, isolated from bone marrow
Host: DH10B
Vector: pCDNA3
Vector type: phagemid
Insert digest: BstXI
Stop Codon Status: without
Description: First-strand cDNA was primed with the oligo-dT primer 5'-AATTCGAGCGGCCGCT(30)VN-3' and a switch primer 5'-TACGGCTGCGAGAAGACGACAGAAGGG-3'. DNA was amplified by 26 cycles of PCR and then size selected for >0.5 kb. An aliquot was used to reamplify for 7 more cycles. The BstXI adaptor 5'-TACGGCTGCGAGAAGACGACAGAAGGG-3' was ligated at both ends and non-directionally cloned into the BstXI site of the pcDNAIII vector. Original library contained 7.5 million clones. Library constructed by Mike Clarke (University of Michigan). Note: this is a NIH_MGC library.

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