Name: NIH_MGC_149 Library ID: 2110 Organism: Mus musculus Strain: C57BL/Ka-Thy1 Age: 0 Stage: embryo Organ: stem cell Tissue: early hematopoietic precursor cells, isolated from bone marrow Host: DH10B Vector: pCDNA3 Vector type: phagemid Insert digest: BstXI Stop Codon Status: without Description: First-strand cDNA was primed with the oligo-dT primer 5'-AATTCGAGCGGCCGCT(30)VN-3' and a switch primer 5'-TACGGCTGCGAGAAGACGACAGAAGGG-3'. DNA was amplified by 26 cycles of PCR and then size selected for >0.5 kb. An aliquot was used to reamplify for 7 more cycles. The BstXI adaptor 5'-TACGGCTGCGAGAAGACGACAGAAGGG-3' was ligated at both ends and non-directionally cloned into the BstXI site of the pcDNAIII vector. Original library contained 7.5 million clones. Library constructed by Mike Clarke (University of Michigan). Note: this is a NIH_MGC library.