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cDNA library: NIH_MGC_150

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Information for 1 library

stem cell

   1. NIH_MGC_150

NIH_MGC_150

Name: NIH_MGC_150
Library ID: 2111
Organism: Mus musculus
Strain: C57BL/Ka-Thy1
Gender: both
Age: 0
Stage: adult
Organ: stem cell
Tissue: multipotent committed hematopoietic progenitor cells
Host: DH10B
Vector: pOTB7-3
Vector type: phagemid
Insert digest: 5' AscI/BstXI 3'
Stop Codon Status: without
Description: Cells were isolated by flow cytometry and RNA isolated in Trizol reagent. cDNA was obtained by reverse transcription using oligo-dT primer 5'-GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV-3'and treated with ExoI. PCR was performed using a complementary 3' primer containing a BstXI site and a cap-switch 5' primer. The resulting cDNA was digested with BstXI and ligated to a BstXI adaptor 5'-AAAAAAAAAAAAAAAAGTCGACATCGATACGCGTGGTCTCGTAGCTGACTTTCCAGCACA-3' then digested with AscI. Size-selection was performed >0.5 kb to result in an average insert size of 0.7 kb. Library constructed and contributed by Mike Clarke and Andrew Hass (University of Michigan). Note: this is an MGC library.

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