Name: NIH_MGC_150 Library ID: 2111 Organism: Mus musculus Strain: C57BL/Ka-Thy1 Gender: both Age: 0 Stage: adult Organ: stem cell Tissue: multipotent committed hematopoietic progenitor cells Host: DH10B Vector: pOTB7-3 Vector type: phagemid Insert digest: 5' AscI/BstXI 3' Stop Codon Status: without Description: Cells were isolated by flow cytometry and RNA isolated in Trizol reagent. cDNA was obtained by reverse transcription using oligo-dT primer 5'-GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV-3'and treated with ExoI. PCR was performed using a complementary 3' primer containing a BstXI site and a cap-switch 5' primer. The resulting cDNA was digested with BstXI and ligated to a BstXI adaptor 5'-AAAAAAAAAAAAAAAAGTCGACATCGATACGCGTGGTCTCGTAGCTGACTTTCCAGCACA-3' then digested with AscI. Size-selection was performed >0.5 kb to result in an average insert size of 0.7 kb. Library constructed and contributed by Mike Clarke and Andrew Hass (University of Michigan). Note: this is an MGC library.