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cDNA library: NIH_MGC_201

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Information for 1 library

embryonic stem cell

   1. NIH_MGC_201

NIH_MGC_201

Name: NIH_MGC_201
Library ID: 2114
Organism: Homo sapiens
Age: 0
Stage: embryo
Organ: embryonic stem cell
Tissue: ES cell line BG01, derived early primative ectodermal like (EPL) cells, the in vitro equivalent of the next pluripotent stage of development
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: Library is oligo-dT primed using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCGAGGCGGCCT(30)-3' and directionally cloned using 5' adaptor sequence: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTATGGCCrGrGrG-3' Average insert size 1.04 kb (range 0.5-2.5 kb). Library is normalized and enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Reference: Biotechniques 30:892-897 (2001). Note: this is a NIH_MGC Library.

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