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cDNA library: NIH_ZGC_15

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Information for 1 library


   1. NIH_ZGC_15


Name: NIH_ZGC_15
Library ID: 2118
Organism: Danio rerio
Strain: wild type
Gender: both
Age: 0
Stage: embryo
Organ: embryo
Tissue: whole embryos staged from 2-8 hr postfertilization, approximately 2500 embryos total
Host: DH10B TonA
Vector: pME18S-FL3
Vector type: phagemid
Insert digest: 5' DraIII/DraIII 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with an oligo(dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of ~1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Note: this is a Zebrafish Gene Collection library.

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