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cDNA library: NIH_BMAP_HJ0

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Information for 1 library

head/neck

   1. NIH_BMAP_HJ0

NIH_BMAP_HJ0

Name: NIH_BMAP_HJ0
Library ID: 2128
Organism: Mus musculus
Strain: C57BL/6
Age: 0
Stage: embryo
Organ: head/neck
Tissue: upper head including skull, brain, and eye (9.5 dpc) and skull and eye (10.5 dpc)
Host: DH10B TonA
Vector: pYX-Asc
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: Non-normalized full-length enriched library from pooled upper head including skull, brain, and eye (9.5 dpc) and skull and eye (10.5 dpc) (size selected for 2-3 kb). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first- synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCCGAACTGAATTTTTTTTTTTTTTTTTT-3') contains the sequence tag CGAACTGAAT between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.

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