Name: NIH_BMAP_HJ0 Library ID: 2128 Organism: Mus musculus Strain: C57BL/6 Age: 0 Stage: embryo Organ: head/neck Tissue: upper head including skull, brain, and eye (9.5 dpc) and skull and eye (10.5 dpc) Host: DH10B TonA Vector: pYX-Asc Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: Non-normalized full-length enriched library from pooled upper head including skull, brain, and eye (9.5 dpc) and skull and eye (10.5 dpc) (size selected for 2-3 kb). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first- synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCCGAACTGAATTTTTTTTTTTTTTTTTT-3') contains the sequence tag CGAACTGAAT between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
