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cDNA library: NIH_MGC_189

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Information for 1 library

thyroid

   1. NIH_MGC_189

NIH_MGC_189

Name: NIH_MGC_189
Library ID: 2133
Organism: Mus musculus
Strain: wild type
Gender: both
Age: 0
Stage: juvenile
Organ: thyroid
Tissue: 5 pooled
Host: DH10B TonA
Vector: pExpress-1
Vector type: plasmid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: RNA obtained from 5 normal wild-type mice. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection 1.4 kb resulted in an average insert size of 1.2 kb. This primary, nanoquantity library is normalized to Cot5 (non-normalized primary library is NIH_MGC_230) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library

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