Name: NIH_ZGC_20 Library ID: 2159 Organism: Danio rerio Strain: wild type AB Gender: both Age: 0 Stage: juvenile Host: DH10B TonA Vector: pME18S-FL3 Vector type: phagemid Insert digest: 5' DraIII/DraIII 3' Stop Codon Status: without Description: 1st strand cDNA was primed with an oligo(dT) primer [GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [GGCCUACUGG], digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for 1.0 kb, with a average insert size of ~1.2kb. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Note: the tissue for this library was originally annotated as pharyngeal arch; however upon sequence analysis it is suspected that this is not the correct tissue source. We have changed tissue to unknown source. Note: this is a Zebrafish Gene Collection library.