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cDNA library: NIH_MGC_260

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Information for 1 library

embryonic stem cell

   1. NIH_MGC_260

NIH_MGC_260

Name: NIH_MGC_260
Library ID: 2187
Organism: Homo sapiens
Gender: male
Age: 0
Stage: embryo
Organ: embryonic stem cell
Tissue: Embryonic stem cells isolated from the inner cell mass of blastocyst stage embryos. Markers: SSEA1-, SSEA3+, SSEA4+, Tra 1-60+, Tra 1-81+, CD9+, Alk Phos+, Oct4+, Nanog+. Passage number: 21
Host: DH10B TonA
Vector: pExpress-1
Vector type: plasmid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: cDNA was primed using oligo-dT primer: 5''-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 1.9 kb. This primary library is not normalized (normalized primary library is NIH_MGC_261) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.

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