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cDNA library: NIH_MGC_269

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Information for 1 library

placenta

   1. NIH_MGC_269

NIH_MGC_269

Name: NIH_MGC_269
Library ID: 2193
Organism: Rattus norvegicus
Strain: Brown Norway line 3
Gender: neither
Age: 0
Stage: placental
Organ: placenta
Tissue: whole placenta, 2 pooled
Host: DH10B TonA
Vector: pExpress-1
Vector type: plasmid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: Tissue was collected from two pooled placentas from the 21st day of pregnancy. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.25 kb fragments for an average insert size of 2.05 kb. A normalized version of this library is also available (NIH_MGC_270). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Mammalian Gene Collection library.

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