Name: NIH_MGC_270 Library ID: 2194 Organism: Rattus norvegicus Strain: Brown Norway line 3 Gender: neither Age: 0 Stage: placental Organ: placenta Tissue: whole placenta, 2 pooled Host: DH10B TonA Vector: pExpress-1 Vector type: plasmid Insert digest: 5' EcoRV/NotI 3' Stop Codon Status: without Description: Tissue was collected from two pooled placentas from the 21st day of pregnancy. 1st strand cDNA was primed with a Not I - oligo(dT) primer, double-stranded cDNA was cloned into the Not I and EcoRV sites of pExpress-1. Library was size-selected for >1.25 kb fragments for an average insert size of 2.15 kb. Library was normalized to Cot7. A non-normalized version of this library is also available (NIH_MGC_269). Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Mammalian Gene Collection library.