Name: NIH_XGC_tropKid1 Library ID: 2202 Organism: Xenopus tropicalis Strain: Nigerian 6th generation inbred Gender: both Age: 0 Stage: adult Organ: kidney Tissue: normal kidney, pool of 14 Host: DH10B TonA Vector: pCS107 Vector type: plasmid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Primers for sequencing: t3 or t7 for 3' end, sp6 for 5' end. Library constructed by Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). Note: this is a Xenopus Gene Collection library.
