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cDNA library: NIH_XGC_tropKid1

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Information for 1 library

kidney

   1. NIH_XGC_tropKid1

NIH_XGC_tropKid1

Name: NIH_XGC_tropKid1
Library ID: 2202
Organism: Xenopus tropicalis
Strain: Nigerian 6th generation inbred
Gender: both
Age: 0
Stage: adult
Organ: kidney
Tissue: normal kidney, pool of 14
Host: DH10B TonA
Vector: pCS107
Vector type: plasmid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Primers for sequencing: t3 or t7 for 3' end, sp6 for 5' end. Library constructed by Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). Note: this is a Xenopus Gene Collection library.

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