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cDNA library: NIH_MGC_277

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Information for 1 library


   1. NIH_MGC_277


Name: NIH_MGC_277
Library ID: 2209
Organism: Homo sapiens
Age: 0
Organ: mixed
Tissue: 20 tissues, mixed
Host: DH10B TonA
Vector: pPCR-Script Amp SK(+)
Vector type: plasmid
Insert digest: SrfI
Stop Codon Status: without
Description: Library was constructed from RT-PCR products generated from the BD Biosciences Clontech Human Total RNA Master Panel II (consisting of RNAs from 20 different tissues). Product from the RT-PCR was purified using QIAGEN PCR columns and ends were repaired for blunt-end ligation into the SrfI site of pPCR-Script Amp SK(+). Clones are oriented with 5' end nearest the NotI site and 3' end nearest the EcoRI site. Companion library NIH_MGC_244 has clones in the opposite orientation. Reference: Wu et al. (2004, Biotechniques in press). Library constructed at Baylor College of Medicine, Human Genome Sequencing Center. Note: this is a Mammalian Gene Collection library.

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