Name: NIH_MGC_277 Library ID: 2209 Organism: Homo sapiens Age: 0 Organ: mixed Tissue: 20 tissues, mixed Host: DH10B TonA Vector: pPCR-Script Amp SK(+) Vector type: plasmid Insert digest: SrfI Stop Codon Status: without Description: Library was constructed from RT-PCR products generated from the BD Biosciences Clontech Human Total RNA Master Panel II (consisting of RNAs from 20 different tissues). Product from the RT-PCR was purified using QIAGEN PCR columns and ends were repaired for blunt-end ligation into the SrfI site of pPCR-Script Amp SK(+). Clones are oriented with 5' end nearest the NotI site and 3' end nearest the EcoRI site. Companion library NIH_MGC_244 has clones in the opposite orientation. Reference: Wu et al. (2004, Biotechniques in press). Library constructed at Baylor College of Medicine, Human Genome Sequencing Center. Note: this is a Mammalian Gene Collection library.