Name: NIH_XGC_tropSkeMus1 Library ID: 2215 Organism: Xenopus tropicalis Strain: Nigerian 6th generation inbred Gender: both Age: 0 Stage: adult Organ: muscle Tissue: skeletal muscle, pooled from 14 individuals Host: DH10B TonA Vector: pCS107 Vector type: plasmid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).