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cDNA library: NIH_MGC_281

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Information for 1 library


   1. NIH_MGC_281


Name: NIH_MGC_281
Library ID: 2220
Organism: Homo sapiens
Gender: female
Age: 0
Stage: embryo
Organ: Blastocyst
Tissue: pluripotent cell line derived from blastocyst inner cell mass
Host: DH10B TonA
Vector: pExpress-1
Vector type: plasmid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: RNA obtained from pluripotent cell line derived from blastocyst inner cell mass (cell line HSF-6, NIH Registry designation UC06. Positive for OCT4 expression by rtPCR, positive for SSEA-3, SSEA-4, Tra-1-81, Tra-1-60 by immunofluorescence. Negative for SSEA-1 by immunofluorescence Passage 62. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 2.0 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_280) and was constructed by Express Genomics (Frederick, MD). Note: this is a Mammalian Gene Collection library.

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