Name: GC_BGC-01 Library ID: 2221 Organism: Bos taurus Strain: Crossbred x Angus Gender: female Age: 0 Stage: juvenile Organ: Peyer's patch Host: DH10B TonA Vector: pBluescript II SK+ Vector type: phagemid Insert digest: 5' XhoI/BamHI 3' Stop Codon Status: without Description: Library is oligo-dT primed using BamHI primer 5'-GAGAGAGAGAAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN-3'. Cap structure is biotinylated and Cap Trapper approach is used to select putative full-length cDNA. cDNA is subjected to oligo-dG tailing followed by second-strand synthesis using XhoI primer 5'-GAGAGAGAGAGAGATTCTTCGAGTTAATTAAATTAATCCCCCCCCCCCCC-3'. After restriction digest, cDNA is directionally cloned into pBluescript II SK+ vector. Library is size selected >400 bp with average size 890 bp and is amplified once. Reference for library construction method: Methods in Enzymology 303:19-45. Sequencing primers: M13R (3') and M13F (5'). Library constructed by J. Yu and S. Moore (University of Alberta, Canada) for the Bovine Genome Sequencing Program.