Name: NIH_XGC_tropGas7 Library ID: 2222 Organism: Xenopus tropicalis Age: 0 Stage: embryo Organ: embryo Tissue: gastrula (stage 10.5-12.5) Host: ElectroTen-Blue Vector: pCS108 Vector type: plasmid Insert digest: 5' SalI/NotI 3' Stop Codon Status: without Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).
