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cDNA library: NIH_XGC_tropGas7

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Information for 1 library

embryo

   1. NIH_XGC_tropGas7

NIH_XGC_tropGas7

Name: NIH_XGC_tropGas7
Library ID: 2222
Organism: Xenopus tropicalis
Age: 0
Stage: embryo
Organ: embryo
Tissue: gastrula (stage 10.5-12.5)
Host: ElectroTen-Blue
Vector: pCS108
Vector type: plasmid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).

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