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cDNA library: NIH_XGC_tropGas6

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Information for 1 library

embryo

   1. NIH_XGC_tropGas6

NIH_XGC_tropGas6

Name: NIH_XGC_tropGas6
Library ID: 2227
Organism: Xenopus tropicalis
Strain: Nigerian 6th generation inbred
Age: 0
Stage: embryo
Organ: embryo
Tissue: gastrula (stage 10-13)
Host: TOP10
Vector: pCS22+
Vector type: plasmid
Insert digest: 5' PstI/XhoI 3'
Stop Codon Status: without
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGCTCT(20)V-3'). After ligation of PstI adapters and XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

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