Name: NIH_XGC_tropNeu5 Library ID: 2229 Organism: Xenopus tropicalis Strain: Nigerian 6th generation inbred Age: 0 Stage: embryo Organ: embryo Tissue: neurula (stage 14-18) Host: TOP10 Vector: pCS22+ Vector type: plasmid Insert digest: 5' PstI/XhoI 3' Stop Codon Status: without Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGCTCT(20)V-3'). After ligation of PstI adapters and XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).
