Name: NIH_XGC_tropHrt1 Library ID: 2233 Organism: Xenopus tropicalis Strain: Nigerian 6th generation inbred Age: 0 Stage: adult Organ: heart Host: DH10B TonA Vector: pCS107 Vector type: plasmid Insert digest: 5' EcoRI/XhoI 3' Stop Codon Status: without Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1678 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).