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cDNA library: NIH_XGC_tropBrn3

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Information for 1 library

brain/CNS

   1. NIH_XGC_tropBrn3

NIH_XGC_tropBrn3

Name: NIH_XGC_tropBrn3
Library ID: 2247
Organism: Xenopus tropicalis
Age: 0
Stage: adult
Organ: brain/CNS
Tissue: whole brain
Host: DH10B
Vector: pCMV-SPORT6
Vector type: phagemid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.

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