Name: NIH_ZGC_22 Library ID: 2258 Organism: Danio rerio Strain: AB Gender: both Age: 0 Stage: adult Organ: gill Tissue: 40 pooled samples Host: DH10B TonA Vector: pME18S-FL3 Vector type: phagemid Insert digest: 5' DraIII/DraIII 3' Stop Codon Status: without Description: 1st strand cDNA was primed with an oligo(dT) primer 5'-GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to a DraIII adaptor 5'-GGCCUACUGG-3', digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for >1.0 kb, with a average insert size of ~1.2 kb and is not amplified. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Reference for library construction: Methods Mol Bio 221:73-91. Note: this is a Zebrafish Gene Collection library.