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cDNA library: NIH_ZGC_22

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Information for 1 library


   1. NIH_ZGC_22


Name: NIH_ZGC_22
Library ID: 2258
Organism: Danio rerio
Strain: AB
Gender: both
Age: 0
Stage: adult
Organ: gill
Tissue: 40 pooled samples
Host: DH10B TonA
Vector: pME18S-FL3
Vector type: phagemid
Insert digest: 5' DraIII/DraIII 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with an oligo(dT) primer 5'-GCGGCTGAAGACGGCCTATGTGGCCTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to a DraIII adaptor 5'-GGCCUACUGG-3', digested and directionally cloned into distinct DraIII sites of the pME18S-FL3. Library was size selected for >1.0 kb, with a average insert size of ~1.2 kb and is not amplified. Library constructed by Yutaka Suzuki (University of Tokyo Institute of Medical Science). Custom primers recommended for sequencing: 5' end primer 5'-GGATGTTGCCTTTACTTCTA-3' and 3' end primer 5'-CGACCTGCAGCTCGAGCACA-3'. Reference for library construction: Methods Mol Bio 221:73-91. Note: this is a Zebrafish Gene Collection library.

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