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cDNA library: GC_BGC-03

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Information for 1 library

mammary gland

   1. GC_BGC-03


Name: GC_BGC-03
Library ID: 2259
Organism: Bos taurus
Strain: Hereford
Gender: female
Age: 4
Stage: adult
Organ: mammary gland
Host: DH10B TonA
Vector: pCMV-SPORT6
Vector type: phagemid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed using 5'-GAGAGAGAGAAAGCGGCCGCAAGAGCTCTTTTTTTTTTTTTTTTVN-3'. Cap structure is biotinylated and Cap Trapper approach is used to select putative full-length cDNA. cDNA is subjected to oligo-dG tailing followed by second-strand synthesis using 5'-GAGAGAGAGAGAGATTGTCGACTTAATTAAATTAATCCCCCCCCCCCCC-3'. After restriction digest, cDNA is directionally cloned into pCMV-SPORT6 vector. Library is size selected >400 bp with average size 1300 bp and is amplified once. Reference for library construction method: Methods in Enzymology 303:19-45. Sequencing primers: M13R (5') and M13F (3'). Library constructed in the laboratory of S. Moore (University of Alberta, Canada) for the Bovine Genome Sequencing Program.

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