Name: NIH_MGC_312 Library ID: 2294 Organism: Homo sapiens Age: 0 Organ: brain/CNS Tissue: cerebellum Host: DH10B TonA Vector: pCR-XL-TOPO Vector type: plasmid Insert digest: EcoRI Stop Codon Status: without Description: Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_313 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
