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cDNA library: NIH_MGC_330

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Information for 1 library

mixed

   1. NIH_MGC_330

NIH_MGC_330

Name: NIH_MGC_330
Library ID: 2306
Organism: Homo sapiens
Age: 0
Organ: mixed
Tissue: cDNA derived from either pooled cytoplasmic polyA RNA from 30 cells lines or pooled total RNA from 10 different tissues (from BD Biosciences/Clontech and Washington University).
Host: DH10B TonA
Vector: pCR-BluntII-TOPO
Vector type: plasmid
Insert digest: TOPO sites
Stop Codon Status: without
Description: Clones from this library have been PCR-amplified using gene-specific primers to contain the complete open reading frame (based on known gene sequences available from NCBI's RefSeq). Template for PCR is cDNA derived from either pooled cytoplasmic polyA RNA from 30 cells lines or pooled total RNA from 10 different tissues (from BD Biosciences/Clontech and Washington University). PCR products are non-directionally cloned into the TOPO sites of the pCR-BluntII-TOPO vector. Library constructed by Dr. Narayan Bhat, Earl Bere III and Hongling Liao (Gene Expression Laboratory, Research Technology Program, SAIC Frederick, NCI-Frederick, Frederick, MD 21702). For information on which gene each clone represents, and the orientation in which it's cloned (determined by sequence analysis; this library represents the forward direction and NIH_MGC_332 represents the reverse direction) please visit our anonymous ftp site here. Note: this is a NIH_MGC Library.

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