Name: GC_BGC-22 Library ID: 2320 Organism: Bos taurus Strain: Hereford Gender: male Age: 0 Stage: fetal Organ: brain/CNS Tissue: cerebellum Host: DH10B TonA Vector: pCMV-SPORT6 Vector type: phagemid Insert digest: 5' SalI/NotI 3' Stop Codon Status: without Description: Library is oligo-dT primed using 5'-GAGAGAGAGAAAGCGGCCGCAAGAGCTCTTTTTTTTTTTTTTTTVN-3'. Cap structure is biotinylated and Cap Trapper approach is used to select putative full-length cDNA. cDNA is subjected to oligo-dG tailing followed by second-strand synthesis using 5'-GAGAGAGAGAGAGATTGTCGACTTAATTAAATTAATCCCCCCCCCCCCC-3'. After restriction digest, cDNA is directionally cloned into pCMV-SPORT6 vector. Library is size selected >500 bp with average size 1.4 kb and is amplified once. Reference for library construction method: Methods in Enzymology 303:19-45. Sequencing primers: M13R (5') and M13F (3'). Library constructed in the laboratory of S. Moore (University of Alberta, Canada) for the Bovine Genome Sequencing Program.
