I.M.A.G.E. Consortium
“Sharing resources to achieve a common goal — the discovery of all genes”
cDNA library: NIH_XGC_tropMet7
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Information for 1 library
embryo
1.
NIH_XGC_tropMet7
NIH_XGC_tropMet7
Name:
NIH_XGC_tropMet7
Library ID:
2366
Organism:
Xenopus tropicalis
Age:
0
Stage:
embryo
Organ:
embryo
Tissue:
metamorphic, stage 62
Host:
DH10B
Vector:
pCMV-SPORT6
Vector type:
phagemid
Insert digest:
5' SalI/NotI 3'
Stop Codon Status:
without
Description:
PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis (1.5-2.5 kb fraction; NIH_XGC_tropMet8 contains the 0.5-1.5 kb fraction) and ligated into SalI and NotI digested pCMV-SPORT6 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute.
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Email address:
Biological Questions and Comments to
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