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cDNA library: NIH_XGC_tropMet9

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Information for 1 library

embryo

   1. NIH_XGC_tropMet9

NIH_XGC_tropMet9

Name: NIH_XGC_tropMet9
Library ID: 2368
Organism: Xenopus tropicalis
Age: 0
Stage: embryo
Organ: embryo
Tissue: metamorphic, stage 64
Host: DH10B
Vector: pCMV-SPORT6
Vector type: phagemid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis (1.5-2.5 kb fraction; NIH_XGC_tropMet10 contains the 0.5-1.5 kb fraction) and ligated into SalI and NotI digested pCMV-SPORT6 vector. Primary library, not amplified. Library constructed at the DOE Joint Genome Institute.

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