Name: NICHD_XGC_limb Library ID: 2408 Organism: Xenopus laevis Strain: wild type Gender: both Age: 0 Stage: juvenile Organ: limb Tissue: hind limb Host: DH10B TonA Vector: pCS111 Vector type: plasmid Insert digest: 5' SmaI/NotI 3' Stop Codon Status: without Description: cDNA was primed using oligo-dT primer: GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25 and cloned into the SmaI/NotI sites of pCS111. Size-selection >900 bp resulted in an average insert size of 1.1 kb. This primary library was constructed by Express Genomics (Frederick, MD). Use M13(-21) primer for 3' end reads, and m13rp for 5' end reads. Note: this is a Xenopus Gene Collection library.