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cDNA library: Soares NbME13.5-14.5

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Information for 1 library

embryo

   1. Soares NbME13.5-14.5

Soares NbME13.5-14.5

Name: Soares NbME13.5-14.5
Library ID: 241
Organism: Mus musculus
Strain: C57BL/6
Age: 0
Stage: fetal
Organ: embryo
Tissue: four pooled embryos
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTTT 3'], on equal amounts of mRNA from 2 13.5dpc and 2 14.5dpc embryos [total RNA provided by Minoru Ko, Wayne State Univ., from 2 ]; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.

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