Name: Soares NbME13.5-14.5 Library ID: 241 Organism: Mus musculus Strain: C57BL/6 Age: 0 Stage: fetal Organ: embryo Tissue: four pooled embryos Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGAAATTTTTTTTTTTTTTTTTTTTTTTTT 3'], on equal amounts of mRNA from 2 13.5dpc and 2 14.5dpc embryos [total RNA provided by Minoru Ko, Wayne State Univ., from 2 ]; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.
