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cDNA library: NIH_ZGC_23

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Information for 1 library

testis

   1. NIH_ZGC_23

NIH_ZGC_23

Name: NIH_ZGC_23
Library ID: 2416
Organism: Danio rerio
Strain: X Tu
Gender: male
Age: 0
Stage: adult
Organ: testis
Tissue: whole testes, pooled
Host: DH10B TonA
Vector: pExpress-1
Vector type: plasmid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI (3') and EcoRV (5') sites of pExpress-1 (EcoRV site destroyed upon ligation). Library has an average insert size of 1.7 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Zebrafish Gene Collection library.

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