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cDNA library: NIH_ZGC_34

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Information for 1 library


   1. NIH_ZGC_34


Name: NIH_ZGC_34
Library ID: 2420
Organism: Danio rerio
Strain: AB
Gender: neither
Age: 0
Organ: egg
Tissue: activated eggs, 10-15 min, pooled
Host: DH10B TonA
Vector: pExpress-1
Vector type: plasmid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.7 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Zebrafish Gene Collection library.

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